Chang H, Tashiro K, Hirai M, Ikeda K, Kurokawa K, Fujita T
Fourth Department of Internal Medicine, Faculty of Medicine, Universityof Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Jun 14;223(2):324-8. doi: 10.1006/bbrc.1996.0893.
We report here the identification of a cDNA encoding a human thiazide-sensitive sodium-chloride cotransporter (hTSC) using a PCR-based method. The homology of the hTSC with rat TSC (rTSC) and rat bumetanide-sensitive sodium-potassium-chloride cotransporter (rBSC) was 86% and 64%, respectively, at the nucleotide level, and 92% and 61%, respectively, at the amino acid level. Using fluorescence in situ hybridization (FISH), the hTSC gene has been mapped to chromosome 16q13. Northern blot analysis using polyA+RNA from various human tissues, revealed a major 4.5 kb transcript and a minor 6.5 kb specifically in the kidney, but low level of expression was also observed in small intestine, placenta, prostate, colon, and spleen.
我们在此报告，使用基于聚合酶链反应（PCR）的方法鉴定出一种编码人噻嗪类敏感型氯化钠共转运体（hTSC）的互补脱氧核糖核酸（cDNA）。在核苷酸水平上，hTSC与大鼠TSC（rTSC）和大鼠布美他尼敏感型钠钾氯共转运体（rBSC）的同源性分别为86%和64%，在氨基酸水平上分别为92%和61%。利用荧光原位杂交（FISH）技术，已将hTSC基因定位于16号染色体q13区。使用来自各种人体组织的聚腺苷酸加尾RNA（polyA+RNA）进行的Northern印迹分析显示，有一条主要的4.5 kb转录本和一条次要的6.5 kb转录本，且特异性地存在于肾脏中，但在小肠、胎盘、前列腺、结肠和脾脏中也观察到了低水平的表达。
