Kolb-Bachofen V, Puchta-Teudt N, Egenhofer C
Institute for Immunobiology, Medical Faculty, Heinrich-Heine-University Düsseldorf, Germany.
Glycoconj J. 1995 Apr;12(2):122-7. doi: 10.1007/BF00731355.
We have shown previously that rat liver macrophages (Kupffer cells) express a membrane-bound form of C-reactive protein (mCRP) on their surface which is identical to a galactose-specific particle receptor activity. We now establish the presence of mCRP on human monocyte-macrophages using immunocytochemistry with an anti-neoCRP specific monoclonal antibody and RNA-RNA in situ hybridization to demonstrate the presence of CRP-specific mRNA. Concomitant with mCRP expression, cells exhibit galactose-dependent uptake of particles coated with lactosylated bovine serum albumin. Adhesion experiments on fibronectin-coated surfaces that mCRP on human blood monocytes may act as a selectin-like adhesion molecule, mediating initial carbohydrate-specific contacts which are followed by peptide-specific recognition via integrin receptors.
我们之前已经表明，大鼠肝巨噬细胞（库普弗细胞）在其表面表达一种膜结合形式的C反应蛋白（mCRP），它与半乳糖特异性颗粒受体活性相同。我们现在使用抗新CRP特异性单克隆抗体的免疫细胞化学和RNA-RNA原位杂交来确定人单核巨噬细胞上mCRP的存在，以证明CRP特异性mRNA的存在。与mCRP表达同时，细胞表现出对乳糖基化牛血清白蛋白包被颗粒的半乳糖依赖性摄取。在纤连蛋白包被表面上的黏附实验表明，人血单核细胞上的mCRP可能作为一种类选择素黏附分子，介导最初的碳水化合物特异性接触，随后通过整合素受体进行肽特异性识别。
