Melnikov Ivan, Kozlov Sergey, Saburova Olga, Zubkova Ekaterina, Guseva Olga, Domogatsky Sergey, Arefieva Tatiana, Radyukhina Natalia, Zvereva Maria, Avtaeva Yuliya, Buryachkovskaya Lyudmila, Gabbasov Zufar
National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
State Research Center of the Russian Federation-Institute of Biomedical Problems of Russian Academy of Sciences, Khoroshevskoye Shosse, Moscow 123007 76A, Russia.
Biomedicines. 2020 Oct 19;8(10):435. doi: 10.3390/biomedicines8100435.
The objective of this work was to study the ability of blood cells and their microparticles to transport monomeric and pentameric forms of C-reactive protein (mCRP and pCRP) in the blood of patients with coronary artery disease (CAD). Blood was obtained from 14 patients with CAD 46 ± 13 years old and 8 healthy volunteers 49 ± 13.6 years old. Blood cells and microparticles with mCRP and pCRP on their surface were detected by flow cytometry. Messenger RNA (mRNA) of CRP was extracted from peripheral blood monocytes stimulated with lipopolysaccharide (LPS) and granulocyte-macrophage colony-stimulating factor (GM-CSF). mRNA of CRP in monocytes was detected with PCR. Monocytes were predominantly pCRP-positive (92.9 ± 6.8%). mCRP was present on 22.0 ± 9.6% of monocyte-derived exosomes. mCRP-positive leukocyte-derived microparticle counts were significantly higher (8764 ± 2876/µL) in the blood of patients with CAD than in healthy volunteers (1472 ± 307/µL). LPS and GM-CSF stimulated monocytes expressed CRP mRNA transcripts levels (0.79 ± 0.73-fold), slightly lower relative to unstimulated hepatocytes of the HepG2 cell line (1.0 ± 0.6-fold), but still detectable. The ability of monocytes to transport pCRP in blood flow, and monocyte-derived exosomes to transmit mCRP, may contribute to the maintenance of chronic inflammation in CAD.
这项工作的目的是研究血细胞及其微粒在冠状动脉疾病（CAD）患者血液中运输单体和五聚体形式的C反应蛋白（mCRP和pCRP）的能力。从14名年龄为46±13岁的CAD患者和8名年龄为49±13.6岁的健康志愿者身上采集血液。通过流式细胞术检测表面带有mCRP和pCRP的血细胞和微粒。从用脂多糖（LPS）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）刺激的外周血单核细胞中提取C反应蛋白的信使核糖核酸（mRNA）。用聚合酶链反应（PCR）检测单核细胞中CRP的mRNA。单核细胞主要为pCRP阳性（92.9±6.8%）。mCRP存在于22.0±9.6%的单核细胞衍生外泌体上。CAD患者血液中mCRP阳性白细胞衍生微粒计数（8764±2876/µL）显著高于健康志愿者（1472±307/µL）。LPS和GM-CSF刺激的单核细胞表达CRP mRNA转录水平（0.79±0.73倍），相对于未刺激的HepG2细胞系肝细胞（1.0±0.6倍）略低，但仍可检测到。单核细胞在血流中运输pCRP以及单核细胞衍生外泌体传递mCRP的能力，可能有助于维持CAD中的慢性炎症。
