Sonesson B, Eide S, Ringborg U, Rorsman H, Rosengren E
Department of Dermatology, University of Lund, University Hospital, Sweden.
Melanoma Res. 1995 Apr;5(2):113-6. doi: 10.1097/00008390-199504000-00007.
We have recently described a new method for measurement of tyrosinase activity in small amounts of human serum (100 microliters), where the purification of tyrosinase is obtained by adsorption of the enzyme to concanavalin A sepharose. The method, which measures stereospecific dopa oxidation, was used in the winter of 1992-93 for the measurement of activity in serum obtained from 30 healthy subjects and from 10 patients with melanoma metastases. The serum tyrosinase values in the 30 subjects ranged from 0.1 to 1.0 nkatal/l, and the mean value and standard deviation was 0.4 +/- 0.2 nkatal/l. In the 10 patients with melanoma, the tyrosinase serum values ranged from 1.1 to 10.6 nkal/l, and the mean value was 3.1 nkatal/l.
我们最近描述了一种测量少量人血清（100微升）中酪氨酸酶活性的新方法，该方法通过将酶吸附到伴刀豆球蛋白A琼脂糖上来纯化酪氨酸酶。这种测量立体特异性多巴氧化的方法在1992 - 1993年冬季用于测量30名健康受试者和10名黑色素瘤转移患者的血清活性。30名受试者的血清酪氨酸酶值在0.1至1.0纳 Katal/升之间，平均值和标准差为0.4±0.2纳 Katal/升。在10名黑色素瘤患者中，血清酪氨酸酶值在1.1至10.6纳 Katal/升之间，平均值为3.1纳 Katal/升。
