使用新型算法GelScann(1.0)准确测定琼脂糖凝胶中的DNA。
Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0).
作者信息
Metzker M L, Allain K M, Gibbs R A
机构信息
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
Comput Appl Biosci. 1995 Apr;11(2):187-94. doi: 10.1093/bioinformatics/11.2.187.
GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann (1.0) uses a statistical method to discriminate DNA from local background fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann (1.0)'s option box. We demonstrate that GelScann(1.0) can accurately determine DNA that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing.
GelScann(1.0)是一款用户友好型程序，可对电荷耦合器件(CCD)成像的琼脂糖凝胶中的DNA进行精确定量。该算法能自动定位泳道，在给定泳道内定位条带，并对每个条带的强度进行定量。GelScann(1.0)使用一种统计方法从局部背景荧光中区分出DNA。用户可通过与GelScann(1.0)的选项框交互来调整泳道查找器和条带查找器的灵敏度。我们证明，GelScann(1.0)能够准确测定DNA浓度，适用于常规分子生物学应用，还能确定用于基因检测的PCR扩增片段的相对强度。
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