Burland V, Curtis F P, Kusukawa N
FMC BioProducts, Rockland, ME, USA.
Biotechniques. 1996 Jul;21(1):142-4. doi: 10.2144/96211pf01.
Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.
