饱和脂肪酸增强轮状病毒的感染性。

Enhancement of rotavirus infectivity by saturated fatty acids.

作者信息

Superti F, Marziano M L, Donelli G, Marchetti M, Seganti L

机构信息

Department of Ultrastructures, Istituto Superiore di Sanita, Rome, Italy.

出版信息

Comp Immunol Microbiol Infect Dis. 1995 Feb;18(2):129-35. doi: 10.1016/0147-9571(95)98854-b.

DOI:10.1016/0147-9571(95)98854-b

PMID:7621669

Abstract

The effect of different saturated fatty acids from 10 to 16 carbon atom chains and some derivatives on the infectivity of SA-11 rotavirus was examined. Both fatty acids and derivatives induced an increase of rotavirus infected LLC-MK2 cells when present during viral absorption to host cells. Capric acid and palmitic acid were the most effective with a dose-dependent relationship. These last lipids, in the same experimental conditions, failed to restore the susceptibility to infection of LLC-MK2 cells made resistant by neuraminidase treatment or to allow cell infection by non-infectious single-shelled viral particles. Results obtained suggest that the enhancing effect on viral infectivity by saturated fatty acids requires previous binding of rotaviral outer capsid proteins to sialic acid containing cell receptors.

摘要

研究了碳链长度为10至16个碳原子的不同饱和脂肪酸及其一些衍生物对SA - 11轮状病毒感染性的影响。当脂肪酸及其衍生物在病毒吸附宿主细胞过程中存在时，二者均可诱导受轮状病毒感染的LLC - MK2细胞数量增加。癸酸和棕榈酸最为有效，且呈剂量依赖性关系。在相同实验条件下，这些脂质无法恢复经神经氨酸酶处理而产生抗性的LLC - MK2细胞的感染易感性，也不能使非感染性单壳病毒颗粒感染细胞。所得结果表明，饱和脂肪酸对病毒感染性的增强作用需要轮状病毒外 capsid 蛋白预先与含唾液酸的细胞受体结合。