Clark S M, Barnett B B, Spendlove R S
J Clin Microbiol. 1979 Mar;9(3):413-7. doi: 10.1128/jcm.9.3.413-417.1979.
Titers of bovine rotavirus in excess of 10(9) immunofluorescent infectious units per ml of culture fluids have been produced, using trypsin treatment of the virus. Infectivity of preparations of the virus can be increased with as little as 1 ng of trypsin per ml, with maximum increases of 1 to 2 log10 with 1 microgram of trypsin per ml. The virus grows to titers in excess of 10(5) immunofluorescent units per ml in MDBK, LLC-MK2, MA-104, and HeLa cells. When MDBK cells are infected with a multiplicity of infection of 20, maximum yields of cell-associated, trypsin-enhanceable virus are obtained 4 to 8 h postinfection. Maximum yields of cell-free, trypsin-enhanceable virus are produced 16 to 20 h postinfection. The results presented here indicate that trypsin can be used to produce high-titer stocks of bovine rotavirus.
通过对病毒进行胰蛋白酶处理，已产生每毫升培养液中牛轮状病毒滴度超过10⁹免疫荧光感染单位的情况。病毒制剂的感染性可通过每毫升低至1纳克的胰蛋白酶来提高，每毫升1微克胰蛋白酶时最大可提高1至2个对数10。该病毒在MDBK、LLC-MK2、MA-104和HeLa细胞中生长至每毫升超过10⁵免疫荧光单位的滴度。当MDBK细胞以20的感染复数感染时，在感染后4至8小时可获得与细胞相关的、胰蛋白酶增强型病毒的最大产量。在感染后16至20小时产生无细胞的、胰蛋白酶增强型病毒的最大产量。此处给出的结果表明，胰蛋白酶可用于生产高滴度的牛轮状病毒毒株。
