Sileghem M, Naessens J
Trypanosomiasis Programme, International Livestock Research Institute, Nairobi, Kenya.
Eur J Immunol. 1995 Jul;25(7):1965-71. doi: 10.1002/eji.1830250725.
Murine models have suggested that CD8 T cells might play a major parasite-promoting role in African trypanosomiasis. To assess the role of these cells in a natural host environment, we have depleted CD8 cells from Boran cattle in vivo and subsequently infected these animals with Trypanosoma congolense by tsetse fly challenge. Following administration of a mouse monoclonal anti-bovine CD8 antibody, we have been able to achieve a depletion of more than 99.9% in peripheral blood, spleen, prescapular lymph nodes, prefemoral lymph nodes, mesenteric lymph nodes and Peyer's patches. Depletion could be maintained over a 4-5-week infection period. Despite the almost total absence of CD8 cells, no effect whatsoever was observed on parasitemia. In addition, anemia, which is the main factor determining the mean survival time in cattle was not affected by the CD8 depletion.
小鼠模型表明，CD8 T细胞可能在非洲锥虫病中发挥主要的促进寄生虫生长的作用。为了评估这些细胞在自然宿主环境中的作用，我们在体内耗尽了博兰牛的CD8细胞，随后通过采采蝇叮咬使这些动物感染刚果锥虫。在给予小鼠单克隆抗牛CD8抗体后，我们能够使外周血、脾脏、肩胛前淋巴结、股前淋巴结、肠系膜淋巴结和派伊尔结中的CD8细胞减少超过99.9%。在4至5周的感染期内，这种减少情况能够维持。尽管几乎完全没有CD8细胞，但未观察到对寄生虫血症有任何影响。此外，贫血是决定牛平均存活时间的主要因素，它并未受到CD8细胞耗竭的影响。
