Milano E G, Chimenti C
Dipartimento di Biologia Animale e dell'Uomo, Università di Roma La Sapienza, Italia.
Gen Comp Endocrinol. 1995 Feb;97(2):239-49. doi: 10.1006/gcen.1995.1023.
Histology and immunohistochemical reactivity (IR) toward insulin, glucagon, somatostatin14, and pancreatic polypeptide were investigated in the pancreas and in other tissues of Bufo bufo during metamorphosis. Insulin IR cells appeared at the very beginning of metamorphosis among exocrine pancreatic cells rich in yolk platelets, not completely arranged in acini. Less than 1 week later, glucagon, somatostatin14, and pancreatic polypeptide IR cells also appeared. At metamorphic climax, both endocrine and exocrine tissues of the pancreas underwent extensive degeneration, mainly via apoptotic cellular death. At this time the number of IR cells and the intensity of immunohistochemical reactions decreased for all the hormones tested. Pancreatic reorganization began at the end of the climax.
在蟾蜍变态发育过程中，对其胰腺及其他组织进行了组织学检查以及针对胰岛素、胰高血糖素、生长抑素14和胰多肽的免疫组织化学反应（IR）研究。胰岛素IR细胞在变态发育刚开始时出现在富含卵黄小板的外分泌胰腺细胞中，这些细胞并未完全排列成腺泡。不到1周后，胰高血糖素、生长抑素14和胰多肽IR细胞也出现了。在变态发育高峰期，胰腺的内分泌和外分泌组织都经历了广泛的退化，主要是通过细胞凋亡死亡。此时，所有检测激素的IR细胞数量和免疫组织化学反应强度均下降。胰腺重组在高峰期结束时开始。
