Accordi F, Chimenti C
Dipartimento di Biologia Animale e dell'Uomo, Università La Sapienza, Roma, Italy.
J Anat. 2003 Mar;202(Pt 3):309-14. doi: 10.1046/j.1469-7580.2003.00161.x.
Tadpoles of Bufo bufo at the end of premetamorphosis were exposed to the action of retinol diluted in the rearing water at two concentrations: 20 and 40 microM, for 48 h. Assuming that retinol causes growth suppression by cell death induction, the TUNEL reaction for the detection of apoptotic cells was performed on paraffin sections of pancreas. The results showed that retinol induced a significant dose-dependent increase of number of apoptotic cells with respect to controls, in which apoptosis was scarce. Electron microscopic observations of treated specimens showed that the effects of retinol mostly occurred on exocrine cells: condensed crescent-shaped nuclear chromatin, dark cytoplasm with long projections; conversely, mitochondria and endoplasmic reticulum appeared unaffected.
将处于变态前末期的中华大蟾蜍蝌蚪暴露于饲养水中稀释的视黄醇作用下，浓度分别为20微摩尔和40微摩尔，作用48小时。假设视黄醇通过诱导细胞死亡导致生长抑制，对胰腺石蜡切片进行用于检测凋亡细胞的TUNEL反应。结果表明，与凋亡稀少的对照组相比，视黄醇诱导凋亡细胞数量显著呈剂量依赖性增加。对处理后的标本进行电子显微镜观察显示，视黄醇的作用主要发生在外分泌细胞上：核染色质呈浓缩的新月形，细胞质暗黑且有长突起；相反，线粒体和内质网未受影响。
