Khaustova N D
Genetika. 1995 May;31(5):646-51.
Dynamics of the Adh activity and frequencies of alleles AdhF and AdhS were analyzed under selection for delayed senescence. The experiments were performed on Drosophila melanogaster. Lines AdhS cn and AdhF vg and experimental populations cn and vg, selected for an increased duration of reproductive period (late oviposition) were used. Analysis of fertility, longevity, viability and resistance to starvation showed that selection for late oviposition resulted in delayed senescence of flies of the experimental populations. Genetic structure of population vg changed considerably with regard to the Adh locus. This was confirmed by parameters of activity, thermostability and electrophoretic mobility of the enzyme isolated from flies after 30 generations of selection. Analysis of frequencies of the Adh alleles showed that in both selected populations, which initially had different genetic composition, accumulated allele AdhS, which encodes the isozyme that is less active but more resistant to inactivation. Genetic mechanism of delayed senescence in Drosophila is assumed to involve selection at vitally important enzyme loci, including Adh.
在针对延缓衰老进行选择的条件下，分析了乙醇脱氢酶（Adh）活性以及AdhF和AdhS等位基因的频率。实验以黑腹果蝇为材料。使用了AdhS cn和AdhF vg品系以及为延长生殖期（晚期产卵）而选择的实验群体cn和vg。对生育力、寿命、活力和抗饥饿能力的分析表明，晚期产卵选择导致实验群体果蝇的衰老延迟。群体vg在Adh基因座方面的遗传结构发生了显著变化。从经过30代选择后的果蝇中分离出的酶的活性、热稳定性和电泳迁移率参数证实了这一点。对Adh等位基因频率的分析表明，在最初具有不同遗传组成的两个选择群体中，都积累了AdhS等位基因，该等位基因编码的同工酶活性较低但更耐失活。果蝇延缓衰老的遗传机制被认为涉及在包括Adh在内的至关重要的酶基因座上进行选择。
