载脂蛋白AICIII-AIV基因簇：载脂蛋白CIII-载脂蛋白AIV基因间区域的序列

The apolipoprotein AICIII-AIV gene cluster: sequence of the ApoCIII-ApoAIV intergenic region.

作者信息

Omori K, Vergnes L, Zakin M M, Ochoa A

机构信息

Unité d'Expression des Gènes Eucaryotes, Institut Pasteur, Paris, France.

出版信息

Gene. 1995 Jul 4;159(2):231-4. doi: 10.1016/0378-1119(95)00063-c.

DOI:10.1016/0378-1119(95)00063-c

PMID:7622055

Abstract

The genes coding for human apolipoproteins AI, CIII and AIV are tandemly organised in a cluster on chromosome 11. The sequence of 4 kb of the 6.6-kb ApoCIII-ApoAIV intergenic region was unknown until now. Since different elements involved in the transcriptional regulation of the three genes of the cluster were previously identified in this region, we decided to sequence it. We present here the complete sequence of the region. Its availability will allow study of the transcriptional regulation of ApoCIII and ApoAIV at the molecular level. In addition, the use of PCR to study the polymorphic sites detected in the ApoCIII-ApoAIV intergenic region will now be possible. Some of these sites have been associated with lipid disorders and coronary heart disease.

摘要

编码人类载脂蛋白AI、CIII和AIV的基因在11号染色体上串联排列成一个簇。直到现在，6.6 kb的载脂蛋白CIII - 载脂蛋白AIV基因间区域中4 kb的序列仍不为人知。由于此前已在该区域鉴定出参与该簇三个基因转录调控的不同元件，我们决定对其进行测序。我们在此展示该区域的完整序列。其可用性将有助于在分子水平上研究载脂蛋白CIII和载脂蛋白AIV的转录调控。此外，现在利用聚合酶链反应（PCR）研究在载脂蛋白CIII - 载脂蛋白AIV基因间区域检测到的多态性位点成为可能。其中一些位点与脂质紊乱和冠心病有关。