Cammarata P, Ceccarini C
J Chromatogr. 1979 Jan 21;168(2):395-400. doi: 10.1016/0021-9673(79)80009-6.
Mannose-labeled cellular glycopeptides derived from human diploid fibroblasts (KL-2) were separated into two classes by QAE-Sephadex chromatography. High-voltage paper electrophoresis and Sephadex G-50 chromatography were used to characterize the glycopeptides further. At least five distinct neutral fractions were isolated with molecular weights ranging from 1050 to 2000 daltons. A linear gradient of ammonium acetate eluted a heterogeneous population of acidic glycopeptides. The use of QAE-Sephadex enables a single-step fractionation of both neutral and acidic glycopeptides on one column. The technique appears to be sensitive enough to distinguish growth-dependent alterations between growing and non-growing cells.
从人二倍体成纤维细胞（KL-2）衍生的甘露糖标记的细胞糖肽通过QAE-葡聚糖凝胶色谱法分为两类。使用高压纸电泳和葡聚糖凝胶G-50色谱法进一步表征糖肽。分离出至少五个不同的中性级分，分子量范围为1050至2000道尔顿。醋酸铵的线性梯度洗脱了酸性糖肽的异质群体。使用QAE-葡聚糖凝胶能够在一根柱上对中性和酸性糖肽进行一步分级分离。该技术似乎足够灵敏，能够区分生长细胞和非生长细胞之间依赖生长的变化。
