Arribas J R, Clifford D B, Fichtenbaum C J, Commins D L, Powderly W G, Storch G A
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri, USA.
J Infect Dis. 1995 Aug;172(2):527-31. doi: 10.1093/infdis/172.2.527.
The objective of this study was to correlate cytomegalovirus (CMV) DNA levels in the cerebrospinal fluid (CSF) of subjects with AIDS with clinical and pathologic findings attributable to CMV infection of the central nervous system (CNS). CMV polymerase chain reaction (PCR) was done on serial dilutions of CSF samples from 24 AIDS patients with autopsy-proven CNS disorders. CMV DNA was detected in CSF from 12 of 13 subjects with evidence of CMV infection of the brain or spinal cord but in none of 11 subjects without autopsy evidence of CMV CNS infection. Subjects whose CSF contained > 10(3) CMV DNA molecules/8 microL of CSF had severe CMV CNS disease (e.g., ventriculoencephalitis). PCR appears to be more useful than clinical and neuroradiologic findings for documenting CMV infection of the CNS in patients with AIDS. Quantitation of CMV DNA in CSF shows promise for evaluation of the extent of involvement.
本研究的目的是将艾滋病患者脑脊液（CSF）中的巨细胞病毒（CMV）DNA水平与中枢神经系统（CNS）CMV感染所致的临床和病理表现相关联。对24例经尸检证实患有中枢神经系统疾病的艾滋病患者的脑脊液样本进行系列稀释后进行CMV聚合酶链反应（PCR）。在13例有脑或脊髓CMV感染证据的受试者中，12例脑脊液中检测到CMV DNA，而11例无CMV中枢神经系统感染尸检证据的受试者中均未检测到。脑脊液中CMV DNA分子>10(3)/8微升的受试者患有严重的CMV中枢神经系统疾病（如脑室脑炎）。对于记录艾滋病患者中枢神经系统的CMV感染，PCR似乎比临床和神经放射学表现更有用。脑脊液中CMV DNA的定量分析有望用于评估受累程度。
