Schaluder G G, Dawson G J, Simons J N, Pilot-Matias T J, Gutierrez R A, Heynen C A, Knigge M F, Kurpiewski G S, Buijk S L, Leary T P
Viral Discovery Group, Abbott Laboratories, North Chicago, Illinois 60064, USA.
J Med Virol. 1995 May;46(1):81-90. doi: 10.1002/jmv.1890460117.
Two flavivirus-like genomes have recently been cloned from infectious tamarin (Saguinus labiatus) serum, derived from the human viral hepatitis GB strain, which is known to induce hepatitis in tamarins. In order to study the natural history of GB infections, further transmission studies were carried out in tamarins. Reverse-transcription-polymerase chain reaction and enzyme-linked immunosorbant assays were developed for the detection of RNA and antibodies associated with the two agents, GB virus-A and GB virus-B. The infectivity of both of these agents was demonstrated in tamarins to be filterable through a 0.1 micron filter. Two distinct genomes were identified in the serum of eight of the infected tamarins, while in four tamarins, the genomes were detected independently of each other. Although specific antibodies to the GB virus-B epitopes were detected in the serum of animals inoculated with both agents or GB virus-B alone, antibodies to putative epitopes specific to GB virus-A were not detected in any of the animals. All tamarins inoculated with serum containing GB virus-B exhibited an elevation in liver enzyme levels after inoculation. Elevations of serum liver enzyme levels did not occur when GB virus-A was the only agent detected in the serum. Infection with the original infectious tamarin inoculum conferred protection from reinfection with GB virus-B but not with GB virus-A.
最近从感染性绢毛猴（白唇绢毛猴）血清中克隆出两个黄病毒样基因组，该血清源自人类病毒性肝炎GB株，已知该病毒株可在绢毛猴中诱发肝炎。为了研究GB感染的自然史，在绢毛猴中开展了进一步的传播研究。开发了逆转录聚合酶链反应和酶联免疫吸附测定法，用于检测与两种病原体GB病毒A和GB病毒B相关的RNA和抗体。在绢毛猴中证实这两种病原体的感染性均可通过0.1微米滤膜过滤。在8只受感染绢毛猴的血清中鉴定出两个不同的基因组，而在4只绢毛猴中，基因组是相互独立检测到的。尽管在接种了两种病原体或仅接种GB病毒B的动物血清中检测到了针对GB病毒B表位的特异性抗体，但在任何动物中均未检测到针对GB病毒A假定表位的抗体。所有接种含GB病毒B血清的绢毛猴在接种后肝酶水平均升高。当血清中仅检测到GB病毒A时，血清肝酶水平未升高。用原始感染性绢毛猴接种物感染可使动物获得对GB病毒B再感染的保护，但对GB病毒A无保护作用。
