Nishimura M, Uchida S, Mitsunaga S, Tokunaga K, Mitomi Y, Tadokoro K, Juji T
Department of Research, Japanese Red Cross, Central Blood Center, Tokyo.
Vox Sang. 1995;68(3):164-8. doi: 10.1111/j.1423-0410.1995.tb03919.x.
Posttransfusion graft-versus-host disease (PTGVHD) is known to develop in immunocompetent patients exhibiting clinical symptoms such as erythroderma, fever, liver dysfunction, diarrhea and pancytopenia. It is speculated that transfused blood donors' lymphocytes might recognize the recipients' HLAs as alloantigens. The thus stimulated lymphocytes might proliferate, expand and finally attack the host's immune system or tissues. However, details regarding these expanded donor cells such as: (1) whether they represent one clone or more, (2) the composition of lymphocyte subsets, and (3) the target HLA antigens of recipients, are not clear, since T-cell lines derived from PTGVHD patients have not yet been obtained. The aim of this study is to characterize T-cells responsible for PTGVHD and to identify their target molecules. For that purpose, we attempted to establish T-cell lines derived from a PTGVHD patient. We show that the established T-cell line, proven to be derived from donor lymphocytes, showed a CD4+ phenotype and had cytotoxic activities. Furthermore, we describe that the target of the cytotoxic T-cell line (CTL) is an HLA-DRB1*0405-related molecule of the patient.
已知输血后移植物抗宿主病（PTGVHD）会在具有免疫活性的患者中发生，这些患者会出现诸如红皮病、发热、肝功能障碍、腹泻和全血细胞减少等临床症状。据推测，输血供体的淋巴细胞可能将受者的人类白细胞抗原（HLA）识别为同种异体抗原。如此被激活的淋巴细胞可能会增殖、扩增并最终攻击宿主的免疫系统或组织。然而，关于这些扩增的供体细胞的细节，例如：（1）它们是代表一个克隆还是多个克隆，（2）淋巴细胞亚群的组成，以及（3）受者的靶HLA抗原，尚不清楚，因为尚未获得源自PTGVHD患者的T细胞系。本研究的目的是对导致PTGVHD的T细胞进行特征分析并确定其靶分子。为此，我们试图建立源自一名PTGVHD患者的T细胞系。我们发现，所建立的T细胞系经证实源自供体淋巴细胞，表现出CD4 +表型并具有细胞毒性活性。此外，我们描述了细胞毒性T细胞系（CTL）的靶标是该患者的一种与HLA - DRB1*0405相关的分子。
