Nishimura M, Uchida S, Mitsunaga S, Tokunaga K, Tadokoro K, Juji T
Department of Research, The Japanese Red Cross Central Blood Centre, Tokyo, Japan.
Br J Haematol. 1996 Mar;92(4):1011-3. doi: 10.1046/j.1365-2141.1996.430973.x.
Post-transfusion graft-versus-host disease (PTGVHD) is one of the most severe side-effects of blood transfusion. To characterize the effector cells causing this disease, we cloned lymphocytes from a PTGVHD patient's peripheral blood. T-cell and B-cell clones were established, the origins of which were proven to be transfused donor lymphocytes. It was found that the B cells produced IgG that mediated complement-dependent cytotoxicity to the cells bearing the patient's HLA class II genotype. Our results suggest, for the first time, the involvement of B-cell-produced cytotoxic antibodies directed against patient's HLA class II in the pathogenesis of PTGVHD.
输血后移植物抗宿主病（PTGVHD）是输血最严重的副作用之一。为了鉴定引发该疾病的效应细胞，我们从一名PTGVHD患者的外周血中克隆淋巴细胞。建立了T细胞和B细胞克隆，证实其来源为输入的供体淋巴细胞。结果发现，B细胞产生IgG，该IgG介导对携带患者HLA II类基因型细胞的补体依赖性细胞毒性。我们的结果首次表明，B细胞产生的针对患者HLA II类的细胞毒性抗体参与了PTGVHD的发病机制。
