红细胞抗原亨肖（He；MNS6）的表达：血清学和免疫化学研究。

Expression of the erythrocyte antigen Henshaw (He; MNS6): serological and immunochemical studies.

作者信息

Reid M E, Lomas-Francis C, Daniels G L, Chen V, Shen J, Ho Y C, Hare V, Batts R, Yacob M, Smart E

机构信息

Immunohematology Laboratory, New York Blood Center, N.Y. 10021, USA.

出版信息

Vox Sang. 1995;68(3):183-6. doi: 10.1111/j.1423-0410.1995.tb03924.x.

DOI:10.1111/j.1423-0410.1995.tb03924.x

PMID:7625076

Abstract

Production of murine monoclonal antibodies to the low prevalence MNS antigen Henshaw (He; MNS6) has enabled more detailed study of this antigen. Using these directly hemagglutinating anti-He, red blood cells (RBCs) from 1695 people of African origin were screened in the USA and England. The prevalence of He+ samples among these donors was 2.1%. In Natal, blood samples from 1218 black donors were screened with rabbit anti-He. The prevalence of He+ donors in this population was 7.0%. Immunoblotting confirmed that the He antigen is carried on an erythrocyte membrane component with a molecular mass that is indistinguishable from glycophorin B. Hemagglutination and immunoblotting demonstrated that ten of 56 He+ samples tested more extensively had a reduced expression of the He antigen. The majority of He+ RBCs were S+; those He+ RBC samples that were S-s+ more frequently had a weakened expression of He.

摘要

针对低频率MNS抗原亨肖（He；MNS6）产生的鼠单克隆抗体，使得对该抗原的更详细研究成为可能。利用这些直接血凝的抗He抗体，在美国和英国对1695名非洲裔人群的红细胞（RBC）进行了筛查。这些供血者中He阳性样本的发生率为2.1%。在纳塔尔，用兔抗He对1218名黑人供血者的血样进行了筛查。该人群中He阳性供血者的发生率为7.0%。免疫印迹证实，He抗原存在于一种红细胞膜成分上，其分子量与血型糖蛋白B无法区分。血凝和免疫印迹表明，在56个接受更广泛检测的He阳性样本中，有10个样本的He抗原表达降低。大多数He阳性红细胞为S阳性；那些S阴性s阳性的He阳性红细胞样本中，He表达减弱的情况更为常见。