用于快速基因定位的一组单染色体体细胞杂种的构建。

Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping.

作者信息

Kelsell D P, Rooke L, Warne D, Bouzyk M, Cullin L, Cox S, West L, Povey S, Spurr N K

机构信息

Human Genetic Resources, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts.

出版信息

Ann Hum Genet. 1995 Apr;59(2):233-41. doi: 10.1111/j.1469-1809.1995.tb00743.x.

DOI:10.1111/j.1469-1809.1995.tb00743.x

PMID:7625768

Abstract

We have assembled a panel of monochromosomal somatic cell hybrids for use in gene mapping. DNA from each individual hybrid was used as a probe on normal human metaphases to identify the human chromosome and any fragments by reverse painting. To test the efficiency of the panel PCR amplification of DNA from the monochromosomal somatic cell hybrid panel was used in combination with human specific oligonucleotide primers to assign alpha-catenin (CTNNA1) and p21/WAF1 to chromosomes 5 and 6 respectively. These genes were localized further using hybrids containing specific translocations to 5q11-qter and 6p21 respectively. We also developed primers to enable us to assign 17 ESTs sequenced by the HGMP Resource Centre. The hybrid panel was developed with support of the UK HGMP and the DNA is available to all registered users.

摘要

我们组建了一组单染色体体细胞杂种用于基因定位。将每个个体杂种的DNA用作正常人中期染色体的探针，通过反向绘画来鉴定人类染色体及其任何片段。为了测试该杂种组的效率，将单染色体体细胞杂种组的DNA进行PCR扩增，并结合人类特异性寡核苷酸引物，分别将α-连环蛋白（CTNNA1）和p21/WAF1定位到5号和6号染色体。利用分别含有5q11-qter和6p21特异性易位的杂种进一步定位这些基因。我们还设计了引物，以便能够定位由人类基因组图谱计划资源中心测序的17个EST。该杂种组是在英国人类基因组图谱计划的支持下开发的，其DNA可供所有注册用户使用。