通过全基因组辐射杂种图谱对人类EST进行区域定位。

Regional assignment of human ESTs by whole-genome radiation hybrid mapping.

作者信息

Hayes P D, Schmitt K, Jones H B, Gyapay G, Weissenbach J, Goodfellow P N

机构信息

HGMP Resource Centre, Hinxton, Cambridgeshire, UK.

出版信息

Mamm Genome. 1996 Jun;7(6):446-50. doi: 10.1007/s003359900130.

DOI:10.1007/s003359900130

PMID:8662228

Abstract

The UK HGMP Resource Centre's collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.

摘要

英国人类基因组映射计划资源中心（HGMP Resource Centre）所收集的人类部分互补DNA序列（ESTs），已被检测其是否适合通过聚合酶链式反应（PCR）在一组体细胞杂种上进行映射。利用单染色体杂种组确定了92个ESTs的染色体定位，并且利用一组全基因组辐射杂种（WG-RHs）将45个ESTs的一个子集与遗传标记进行了连锁分析。这些结果证明了WG-RHs构建人类基因组转录图谱的潜力。