Schadendorf D, Kohlmus C, Gawlik C, Suter L, Czarnetzki B M
Department of Dermatology, University Hospital Rudolf Virchow, FU Berlin, Germany.
Arch Dermatol Res. 1995;287(5):452-6. doi: 10.1007/BF00373428.
The importance of mast cells in local homeostasis, inflammation and tumour surveillance is supported by many studies, and the association of mast cells with various neoplasms has been known for a long time. The functional significance of mast cells surrounding tumours is currently unclear. In the present histopathological study, 16 naevi and 44 primary malignant melanomas were analysed. The follow-up time after primary surgery of malignant melanoma patients was almost 10 years, during which time half of the tumours had metastasized. The numbers of mast cells surrounding pigmented lesions were determined. The mast cells were visualized using a modified toluidine blue staining (pH 0.5; 24 h). Significantly different numbers of mast cells were found in naevi compared to melanomas (P = 0.0013), but no significant correlation could be identified regarding prognostic parameters including tumour thickness and clinical outcome.
许多研究都证实了肥大细胞在局部内环境稳定、炎症和肿瘤监测中的重要性，并且肥大细胞与各种肿瘤的关联早已为人所知。目前尚不清楚肿瘤周围肥大细胞的功能意义。在本组织病理学研究中，分析了16个痣和44例原发性恶性黑色素瘤。恶性黑色素瘤患者初次手术后的随访时间接近10年，在此期间一半的肿瘤发生了转移。测定了色素沉着病变周围肥大细胞的数量。使用改良甲苯胺蓝染色法（pH 0.5；24小时）使肥大细胞可视化。与黑色素瘤相比，痣中肥大细胞的数量有显著差异（P = 0.0013），但在包括肿瘤厚度和临床结果在内的预后参数方面未发现显著相关性。
