Smith A J, Cassidy N, Perry H, Bègue-Kirn C, Ruch J V, Lesot H
School of Dentistry, University of Birmingham, United Kingdom.
Int J Dev Biol. 1995 Feb;39(1):273-80.
Reactionary dentinogenesis is the secretion of a tertiary dentine matrix by surviving odontoblast cells in response to an appropriate stimulus. Whilst this stimulus may be exogenous in nature, it may also be from endogenous tissue components released from the matrix during pathological processes. Implantation of isolated dentine extracellular matrix components in unexposed cavities of ferret teeth led to stimulation of underlying odontoblasts and a response of reactionary dentinogenesis. Affinity chromatography of the active components prior to implantation and assay for growth factors indicated that this material contained significant amounts of TGF-beta 1, a growth factor previously shown to influence odontoblast differentiation and secretory behavior. Reactionary dentinogenesis during dental caries probably results from solubilization of growth factors, TGF-beta in particular, from the dentine matrix which then are responsible for initiating the stimulatory effect on the odontoblasts. Compositional differences in tertiary dentine matrices beneath carious lesions in human teeth have also been shown indicating modulation of odontoblast secretion during reactionary and reparative dentinogenesis.
反应性牙本质形成是成牙本质细胞在适当刺激下分泌第三期牙本质基质。虽然这种刺激可能是外源性的，但也可能来自病理过程中从基质释放的内源性组织成分。将分离的牙本质细胞外基质成分植入雪貂牙齿未暴露的窝洞中，会刺激下层的成牙本质细胞并引发反应性牙本质形成。植入前对活性成分进行亲和层析并检测生长因子，结果表明该物质含有大量的转化生长因子β1（TGF-β1），这是一种先前已证明会影响成牙本质细胞分化和分泌行为的生长因子。龋齿过程中的反应性牙本质形成可能是由于牙本质基质中生长因子（特别是TGF-β）的溶解，这些生长因子随后对成牙本质细胞产生刺激作用。研究还表明，人类牙齿龋损下方第三期牙本质基质存在成分差异，这表明在反应性和修复性牙本质形成过程中，成牙本质细胞的分泌受到了调节。
