Thomas G A, Lelkes P I, Chick D M, Lu H, Kowal T A, Hammond R L, Nakajima H, Nakajima H, Spanta A D, Stephenson L W
Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan, USA.
J Card Surg. 1995 May;10(3):245-56. doi: 10.1111/j.1540-8191.1995.tb00605.x.
Twelve bilateral skeletal muscle ventricles (SMVs) were constructed in six dogs by wrapping each latissimus dorsi muscle around a cylindrical, plastic mandrel (volume 30 cc). After 6 to 10 weeks, five dogs had one of their SMVs seeded with allogeneic cultured canine endothelial cells (8 x 10(6) cells/pouch) via an open technique, whil the contralateral SMV was seeded by percutaneous injection of cells into the space around the mandrel. After 1 week, the SMVs were excised. Viable, adherent endothelial cells were present in all seeded pouches; this was confirmed via fluorescent microscopy with several endothelial cell markers; KLH-2, dilacetylated low-density lipoprotein and antibodies to von Willebrand factor. The inner lining of the SMVs were also examined with scanning and transmission electron microscopy; the highest concentration of cells were seen at the apex where a continuous endothelial monolayer was observed. No significant difference in the distribution or the morphology of the endothelial lining was noted between the open and percutaneous seeding techniques. These data show that SMVs can be seeded with an endothelial monolayer using both open and percutaneous techniques.
通过将每块背阔肌包裹在一个圆柱形塑料心轴（容积30立方厘米）周围，在6只狗身上构建了12个双侧骨骼肌心室（SMV）。6至10周后，5只狗通过开放技术将同种异体培养的犬内皮细胞（8×10⁶个细胞/囊袋）接种到其中一个SMV中，而对侧的SMV则通过经皮将细胞注射到心轴周围的空间进行接种。1周后，切除SMV。在所有接种的囊袋中均存在存活的、贴壁的内皮细胞；通过使用几种内皮细胞标记物的荧光显微镜检查证实了这一点；KLH-2、双乙酰化低密度脂蛋白和抗血管性血友病因子抗体。还通过扫描电子显微镜和透射电子显微镜检查了SMV的内衬；在顶端观察到连续内皮单层的地方细胞浓度最高。在开放接种技术和经皮接种技术之间，内皮内衬的分布或形态没有显著差异。这些数据表明，使用开放技术和经皮技术都可以在内皮单层上接种SMV。
