Nehls M, Lüno K, Schorpp M, Pfeifer D, Krause S, Matysiak-Scholze U, Dierbach H, Boehm T
Department of Medicine I, University of Freiburg, Germany.
Mamm Genome. 1995 May;6(5):321-31. doi: 10.1007/BF00364794.
The characterization of three YAC/P1 contigs from adjacent segments of the central region of mouse Chromosome (Chr) 11 is described. These contigs are based upon 63 YACs and 40 P1 recombinants. From these clones, 185 end sequences were obtained, of which 147 sequences could be converted into sequence-tagged sites and mapped within the three contigs. Deletions were detected in 16 out of 63 YACs; 19 of 63 YACs were found to be chimeric. No such aberrations were found in P1 recombinants. A total of 22 public and 34 newly developed microsatellite markers were unambiguously localized to and ordered in the contigs. In the cryb1/Nf1 interval of the central contig, several new genes have been identified by exon trapping and precisely localized with respect to known STS markers.
本文描述了来自小鼠11号染色体（Chr）中心区域相邻片段的三个酵母人工染色体（YAC）/P1重叠群的特征。这些重叠群基于63个YAC和40个P1重组体。从这些克隆中获得了185个末端序列，其中147个序列可转化为序列标签位点并定位在三个重叠群内。在63个YAC中的16个中检测到缺失；63个YAC中的19个被发现是嵌合体。在P1重组体中未发现此类异常。总共22个公共微卫星标记和34个新开发的微卫星标记被明确地定位到重叠群中并进行了排序。在中心重叠群的cryb1/Nf1区间，通过外显子捕获鉴定了几个新基因，并相对于已知的序列标签位点（STS）标记进行了精确定位。
