Brockmann G A, Haley C S, Renne U, Knott S A, Schwerin M
Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany.
Genetics. 1998 Sep;150(1):369-81. doi: 10.1093/genetics/150.1.369.
Quantitative trait loci (QTL) influencing body weight were mapped by linkage analysis in crosses between a high body weight selected line (DU6) and a control line (DUKs). The two mouse lines differ in body weight by 106% and in abdominal fat weight by 100% at 42 days. They were generated from the same base population and maintained as outbred colonies. Determination of line-specific allele frequencies at microsatellite markers spanning the genome indicated significant changes between the lines on 15 autosomes and the X chromosome. To confirm these effects, a QTL analysis was performed using structured F2 pedigrees derived from crosses of a single male from DU6 with a female from DUKs. QTL significant at the genome-wide level were mapped for body weight on chromosome 11; for abdominal fat weight on chromosomes 4, 11, and 13; for abdominal fat percentage on chromosomes 3 and 4; and for the weights of liver on chromosomes 4 and 11, of kidney on chromosomes 2 and 9, and of spleen on chromosome 11. The strong effect on body weight of the QTL on chromosome 11 was confirmed in three independent pedigrees. The effect was additive and independent of sex, accounting for 21-35% of the phenotypic variance of body weight within the corresponding F2 populations. The test for multiple QTL on chromosome 11 with combined data from all pedigrees indicated the segregation of two loci separated by 36 cM influencing body weight.
通过对高体重选择系（DU6）和对照系（DUKs）杂交后代进行连锁分析，定位了影响体重的数量性状基因座（QTL）。这两个小鼠品系在42日龄时体重相差106%，腹部脂肪重量相差100%。它们源自同一个基础群体，作为远交群体维持。对覆盖整个基因组的微卫星标记进行品系特异性等位基因频率测定，结果表明15条常染色体和X染色体上的品系间存在显著变化。为了证实这些效应，利用来自DU6的一只雄性与DUKs的一只雌性杂交产生的结构化F2家系进行了QTL分析。在全基因组水平上显著的QTL被定位到：体重相关的位于11号染色体；腹部脂肪重量相关的位于4号、11号和13号染色体；腹部脂肪百分比相关的位于3号和4号染色体；肝脏重量相关的位于4号和11号染色体，肾脏重量相关的位于2号和9号染色体，脾脏重量相关的位于11号染色体。11号染色体上的QTL对体重的强烈影响在三个独立家系中得到证实。该效应是加性的且与性别无关，占相应F2群体中体重表型变异的21%-35%。对所有家系的合并数据进行的11号染色体上多个QTL的检验表明，存在两个相距36厘摩且影响体重的基因座的分离。
