Kanjilal S, Strom S S, Clayman G L, Weber R S, el-Naggar A K, Kapur V, Cummings K K, Hill L A, Spitz M R, Kripke M L
Department of Immunology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cancer Res. 1995 Aug 15;55(16):3604-9.
Multiple and distinct p53 mutations were detected by DNA sequence analysis in tumor and adjacent nonmalignant skin samples from eight patients with nonmelanoma skin cancer of the head and neck, providing unambiguous evidence for field cancerization. The mutations consisted of C-->T transitions at dipyrimidine sequences (30% of all single base substitutions), T-->C transitions (47%), and G-->T transversions (12%), suggesting that other carcinogens may act along with UV radiation in the development of nonmelanoma skin cancer. Patient interviews revealed that, in addition to substantial exposure to solar UV radiation, most had a history of smoking and were exposed to carcinogens from industrial or agricultural sources. These data show that extensive molecular epidemiological investigations are necessary to elucidate risk factors associated with the disease in localities where patients often report substantial exposure to environmental carcinogens.
通过DNA序列分析，在8例头颈部非黑色素瘤皮肤癌患者的肿瘤及相邻非恶性皮肤样本中检测到多个不同的p53突变，为场癌化提供了明确证据。这些突变包括二嘧啶序列处的C→T转换（占所有单碱基替换的30%）、T→C转换（47%）和G→T颠换（12%），表明在非黑色素瘤皮肤癌的发生过程中，可能有其他致癌物与紫外线辐射共同作用。患者访谈显示，除了大量暴露于太阳紫外线辐射外，大多数患者有吸烟史，且接触过来自工业或农业源的致癌物。这些数据表明，在患者常报告大量接触环境致癌物的地区，有必要进行广泛的分子流行病学调查，以阐明与该疾病相关的危险因素。
