Barton M H, Moore J N
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Circ Shock. 1994 Dec;44(4):216-20.
Whole blood from 10 healthy horses was aseptically collected into heparin or citrate anticoagulant and incubated in vitro for 6 hr in the absence (saline control) or presence of 1 ng endotoxin/ml blood. Pentoxifylline (0.1, 1, 10, or 100 micrograms/ml blood) was added 1 hr before, at the same time, or 1 hr after endotoxin. As compared to saline controls, pentoxifylline alone had no effect on mediator production, with the exception of significantly increasing 6-ketoprostaglandin F1 alpha concentration. Pentoxifylline inhibited endotoxin-induced increases in tumor necrosis factor (TNF) and interleukin-6 (IL-6) activity in a dose-related fashion, whether added before, at the same time, or after endotoxin. Pentoxifylline significantly inhibited tissue factor activity, but only when added before endotoxin. Pentoxifylline had no effect on endotoxin-induced 6-keto-prostaglandin F1 alpha production, but significantly inhibited thromboxane B2 (TxB2) production. The results of this study indicate that pentoxifylline, at blood concentrations consistent with those achieved in vivo, has effects that may be beneficial in the treatment of endotoxemia.
从10匹健康马无菌采集全血，分别置于肝素或柠檬酸盐抗凝剂中，在无内毒素（生理盐水对照）或有1 ng内毒素/ ml血液的情况下于体外孵育6小时。己酮可可碱（0.1、1、10或100微克/ ml血液）在内毒素加入前1小时、同时或加入后1小时添加。与生理盐水对照相比，单独使用己酮可可碱对介质产生无影响，但显著增加6 - 酮前列腺素F1α浓度除外。己酮可可碱以剂量相关方式抑制内毒素诱导的肿瘤坏死因子（TNF）和白细胞介素-6（IL-6）活性增加，无论在内毒素加入前、同时还是加入后添加。己酮可可碱显著抑制组织因子活性，但仅在内毒素加入前添加时有效。己酮可可碱对内毒素诱导的6 - 酮前列腺素F1α产生无影响，但显著抑制血栓素B2（TxB2）产生。本研究结果表明，己酮可可碱在与体内达到的浓度一致的血药浓度下，具有可能有益于治疗内毒素血症的作用。
