Costes V, Marty-Ané C, Picot M C, Serre I, Pujol J L, Mary H, Baldet P
Department of Pathology, Hopital Lapeyronie, Montpellier, France.
Hum Pathol. 1995 Jul;26(7):740-5. doi: 10.1016/0046-8177(95)90221-x.
Expression of a proliferating antigen by KI-67 immunohistochemistry was analyzed with a SAMBA 2005 computer-assisted image processor (Traitement de l'Information for des Techniques Nouvelles, Grenoble, France) in 47 surgically resected bronchopulmonary carcinoids embedded in paraffin. The clinicopathologic characteristics and KI-67 labeling, expressed in percentage of stained nuclear surface relative to the total nuclear surface, of 31 typical carcinoids and 16 atypical carcinoids were compared and assessed with respect to patient survival. The proliferation status was significantly higher in histologically atypical than in typical carcinoids. Moreover, using a 4% cutoff, we observed a significant difference for the 4-year overall survival rate. Semiquantitative analysis of the proliferation index by KI-67 immunostaining seemed to be an effective means of identifying high risk subsets among patients with histologically atypical carcinoids and for whom adjuvant chemotherapy could be proposed.
使用SAMBA 2005计算机辅助图像处理器（法国格勒诺布尔的Traitement de l'Information for des Techniques Nouvelles公司）对47例石蜡包埋的手术切除支气管肺类癌进行KI-67免疫组化分析，以检测增殖抗原的表达。比较并评估31例典型类癌和16例非典型类癌的临床病理特征及KI-67标记（以染色核表面积占总核表面积的百分比表示）与患者生存率的关系。组织学上非典型类癌的增殖状态显著高于典型类癌。此外，以4%为临界值，我们观察到4年总生存率存在显著差异。通过KI-67免疫染色对增殖指数进行半定量分析似乎是识别组织学非典型类癌患者中高危亚组以及建议进行辅助化疗患者的有效方法。
