来自酿酒酵母的三种新小核RNA的特性：snR34、snR35和snR36。

Characterization of three new snRNAs from Saccharomyces cerevisiae: snR34, snR35 and snR36.

作者信息

Samarsky D A, Balakin A G, Fournier M J

机构信息

Department of Biochemistry and Molecular Biology, Lederle Graduate Research Center, University of Massachusetts, Amherst 01002, USA.

出版信息

Nucleic Acids Res. 1995 Jul 11;23(13):2548-54. doi: 10.1093/nar/23.13.2548.

Genes for three novel snRNAs of Saccharomyces cerevisiae have been isolated, sequenced and tested for essentiality. The RNAs encoded by these genes are designated snR34, snR35 and snR36 respectively and contain 203, 204 and 182 nucleotides. Each RNA is derived from a single copy gene and all three RNAs are believed to be nucleolar, i.e. snoRNAs, based on extraction properties and association with fibrillarin. SnR34 and snR35 contain a trimethylguanosine cap, but this feature is absent from snR36. The novel RNAs lack elements conserved among several other snoRNAs, including box C, box D and long sequence complementarities with rRNA. Genetic disruption analyses showed each of the RNAs to be dispensable and a haploid strain lacking all three RNAs and a previously characterized fourth snoRNA (snR33) is also viable. No differences in the levels of precursors or mature rRNAs were apparent in the four gene knock-out strain. Possible roles for the new RNAs in ribosome biogenesis are discussed.

酿酒酵母三种新型小核仁RNA（snRNA）的基因已被分离、测序并进行了必需性测试。这些基因编码的RNA分别命名为snR34、snR35和snR36，分别含有203、204和182个核苷酸。每种RNA都来源于单拷贝基因，基于提取特性以及与纤维蛋白的关联，所有这三种RNA都被认为是核仁的，即小核仁RNA（snoRNA）。SnR34和snR35含有三甲基鸟苷帽，但snR36没有这个特征。这些新型RNA缺乏在其他几种snoRNA中保守的元件，包括C盒、D盒以及与rRNA的长序列互补性。基因敲除分析表明，每种RNA都是非必需的，并且一个缺乏所有三种RNA以及先前已鉴定的第四种snoRNA（snR33）的单倍体菌株也是可存活的。在四基因敲除菌株中，前体或成熟rRNA的水平没有明显差异。文中讨论了这些新RNA在核糖体生物发生中的可能作用。