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一种新型酵母小核仁RNA的鉴定与功能分析。

Identification and functional analysis of a novel yeast small nucleolar RNA.

作者信息

Dandekar T, Tollervey D

机构信息

EMBL, Heidelberg, Germany.

出版信息

Nucleic Acids Res. 1993 Nov 25;21(23):5386-90. doi: 10.1093/nar/21.23.5386.

DOI:10.1093/nar/21.23.5386
PMID:8265353
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310575/
Abstract

snR31 is a RNA species of 225 nt. which has the trimethyl guanosine cap structure typical of small nuclear RNAs (snRNAs) and yeast small nucleolar RNAs (snoRNAs), and is associated with the nucleolar proteins fibrillarin (NOP1) and GAR1. On sub-nuclear fractionation, snR31 behaves like other snoRNAs, and is enriched in a nucleolar fraction. The SNR31 genomic locus is close to the SNR5 locus, which encodes another snoRNA. The two genes are divergently transcribed with 217 bp separating the transcription start sites. Disruption of the SNR31 gene does not detectably impair growth in a haploid strain. Analyses of pre-rRNA processing in wild-type and snr31- strains shows some accumulation of the 35S primary transcript in the mutant, indicating a mild impairment of the initial steps in pre-rRNA processing.

摘要

snR31是一种225个核苷酸的RNA分子，具有典型的小核RNA（snRNA）和酵母小核仁RNA（snoRNA）的三甲基鸟苷帽结构，并且与核仁蛋白纤维蛋白原（NOP1）和GAR1相关联。在亚核分级分离中，snR31的行为与其他snoRNA相似，并且在核仁级分中富集。SNR31基因座靠近SNR5基因座，后者编码另一种snoRNA。这两个基因以相反方向转录，转录起始位点之间相隔217个碱基对。在单倍体菌株中，SNR31基因的破坏并未检测到对生长有明显损害。对野生型和snr31-菌株中前体rRNA加工的分析表明，突变体中35S初级转录本有一些积累，这表明前体rRNA加工的初始步骤有轻度损害。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/9099675d1fa2/nar00072-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/5c2068220868/nar00072-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/a043d6b16626/nar00072-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/9099675d1fa2/nar00072-0110-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/5c2068220868/nar00072-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/a043d6b16626/nar00072-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6792/310575/9099675d1fa2/nar00072-0110-b.jpg

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Identification and functional analysis of a novel yeast small nucleolar RNA.一种新型酵母小核仁RNA的鉴定与功能分析。
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引用本文的文献

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本文引用的文献

1
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.U8核仁小核仁核糖核酸的破坏会抑制非洲爪蟾卵母细胞中5.8S和28S核糖体核糖核酸的加工。
Cell. 1993 Jun 18;73(6):1233-45. doi: 10.1016/0092-8674(93)90651-6.
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A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing.核糖体RNA加工需要一种与剪接因子PRP4和Gβ结构域具有同源性的U3小核核糖核蛋白（snoRNP）蛋白。
EMBO J. 1993 Jun;12(6):2549-58. doi: 10.1002/j.1460-2075.1993.tb05910.x.
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Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
酵母snR30是18S核糖体RNA合成所需的一种小核仁RNA。
Mol Cell Biol. 1993 Apr;13(4):2469-77. doi: 10.1128/mcb.13.4.2469-2477.1993.
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Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly.温度敏感型突变表明酵母纤连蛋白在核糖体前体RNA加工、核糖体前体RNA甲基化和核糖体组装中发挥作用。
Cell. 1993 Feb 12;72(3):443-57. doi: 10.1016/0092-8674(93)90120-f.
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Three small nucleolar RNAs of unique nucleotide sequences.三种具有独特核苷酸序列的小核仁RNA。
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):635-8. doi: 10.1073/pnas.90.2.635.
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A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA.一种新型小核仁RNA（U16）编码于核糖体蛋白内含子内，由前体mRNA加工产生。
EMBO J. 1993 Jul;12(7):2921-8. doi: 10.1002/j.1460-2075.1993.tb05954.x.
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Small nucleolar RNAs encoded by introns of the human cell cycle regulatory gene RCC1.由人类细胞周期调控基因RCC1的内含子编码的小核仁RNA。
EMBO J. 1993 Jul;12(7):2913-20. doi: 10.1002/j.1460-2075.1993.tb05953.x.
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A small nucleolar RNA is processed from an intron of the human gene encoding ribosomal protein S3.一种小核仁RNA是从编码核糖体蛋白S3的人类基因的一个内含子加工而来的。
Genes Dev. 1993 Jul;7(7A):1176-90. doi: 10.1101/gad.7.7a.1176.
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Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
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