Paquet F, Verry M, Grillon G, Landesman C, Masse R, Taylor D M
Laboratoire de Radiotoxicologie, CEA. DSV. DPTE, Bruyères le châtel, France.
Radiat Res. 1995 Aug;143(2):214-8.
The present investigation was aimed at establishing the distribution of 237Np within the different structures of hepatocytes. Rats were contaminated experimentally by intravenous injection of 237Np (V) and the subcellular structures of the liver were separated by ultracentrifugation. Twenty-four hours after contamination, the nuclear and cytosolic fractions bound 54 and 32%, respectively, of the total radionuclide. Purification of the nuclei followed by dissociation of the protein components in medium of increasing ionic strength showed a specific binding of neptunium to the structural proteins of the nuclear matrix.
本研究旨在确定237Np在肝细胞不同结构中的分布情况。通过静脉注射237Np（V）对大鼠进行实验性污染，并用超速离心法分离肝脏的亚细胞结构。污染24小时后，核组分和胞质组分分别结合了总放射性核素的54%和32%。对细胞核进行纯化，然后在离子强度不断增加的介质中使蛋白质成分解离，结果表明镎与核基质的结构蛋白存在特异性结合。
