Alesenko A V, Krasil'nikov V A, Boĭkov P Ia, Loginov A S, Makar'eva E D
Biokhimiia. 1989 Feb;54(2):328-37.
Well coordinated stages of inhibition, restoration and stimulation of protein, DNA and RNA synthesis were observed after administration of cycloheximide (3 mg/kg). The changes in lipid synthesis and composition in the nuclei and intranuclear structures were studied at different stages of cycloheximide action. The accumulation and stimulation of lipid synthesis in the nuclei during the inhibition and restoration of protein and DNA syntheses were followed by electron microscopy and labeled precursors methods. Dramatic changes were observed in the phospholipid composition of chromatin and nuclear matrix. The accumulation of minor phospholipid fractions in intranuclear structures was observed during DNA synthesis. The sphingomyelin concentration was predominant and commensurable with those of phosphatidylcholine and phosphatidylethanolamine.
给予环己酰亚胺（3毫克/千克）后，观察到蛋白质、DNA和RNA合成的抑制、恢复和刺激阶段协调良好。在环己酰亚胺作用的不同阶段，研究了细胞核和核内结构中脂质合成和组成的变化。通过电子显微镜和标记前体方法追踪了蛋白质和DNA合成抑制和恢复过程中细胞核内脂质合成的积累和刺激情况。观察到染色质和核基质的磷脂组成发生了显著变化。在DNA合成过程中，观察到核内结构中次要磷脂组分的积累。鞘磷脂浓度占主导地位，与磷脂酰胆碱和磷脂酰乙醇胺的浓度相当。
