Vencovsky J, Mageed R A, Ollier W E, Maini R N
Kennedy Institute of Rheumatology, Hammersmith, London, UK.
Scand J Immunol. 1995 Jul;42(1):147-57. doi: 10.1111/j.1365-3083.1995.tb03638.x.
The degree of polyclonal RF heterogeneity was assessed in diseased and non-diseased twins with rheumatoid arthritis (RA). The distribution of variable region determinants encoded by a set of immunoglobulin germline, or minimally mutated germline, genes within IgM RF, IgG RF and IgA RF isotypes was determined by ELISA using specific mouse monoclonal antibodies (MoAb) in fractionated plasma from 12 members of six monozygotic twin pairs with RA. The results reveal that at least 40% (range approximately 18-87%) of IgM RF are encoded by a small set of approximately 10 genes from the VH1, 3 and 4 families. Furthermore, a significant proportion of IgG RF and IgA RF (approximately 30%) are also encoded by these same genes. Comparison with RF-negative fractions of immunoglobulins showed that the examined variable region determinants were overrepresented in the RF fractions. The level of expression of the variable region determinants in RF were generally similar within twins but different between unrelated twin pairs irrespective of disease status. The variability of VH gene usage between unrelated individuals suggests that the level of expression and regulation of the variable region determinants may be genetically regulated or influenced by common environmental factors.
在患有和未患类风湿性关节炎（RA）的双胞胎中评估了多克隆RF异质性的程度。通过ELISA，使用特异性小鼠单克隆抗体（MoAb），对来自6对患有RA的单卵双胞胎的12名成员的分级血浆中的IgM RF、IgG RF和IgA RF同种型内的一组免疫球蛋白种系或最小突变种系基因编码的可变区决定簇的分布进行了测定。结果显示，至少40%（范围约为18 - 87%）的IgM RF由来自VH1、3和4家族的约10个基因的一小部分编码。此外，相当一部分IgG RF和IgA RF（约30%）也由这些相同的基因编码。与免疫球蛋白的RF阴性部分进行比较表明，所检测的可变区决定簇在RF部分中过度表达。可变区决定簇在RF中的表达水平在双胞胎内通常相似，但在无关的双胞胎对之间不同，无论疾病状态如何。无关个体之间VH基因使用的变异性表明，可变区决定簇的表达水平和调控可能受遗传调控或受共同环境因素影响。
