方法与试剂。用质粒DNA对大肠杆菌进行电转化。

Methods and reagents. Electro-transformation of Escherichia coli with plasmid DNA.

作者信息

Hengen P N

机构信息

National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

出版信息

Trends Biochem Sci. 1995 Jun;20(6):248-9. doi: 10.1016/s0968-0004(00)89028-3.

DOI:10.1016/s0968-0004(00)89028-3

PMID:7631423

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses some reasons for switching to electroporation to transform bacteria with plasmid DNA. For details on how to partake in the newsgroup, see the accompanying box.

摘要

《方法与试剂》是一个独特的月度专栏，重点介绍互联网上新闻组bionet.molbio.methds - reagnts中当前的讨论内容。本月的专栏讨论了一些将质粒DNA转化细菌的方法转换为电穿孔法的原因。有关如何加入新闻组的详细信息，请参阅随附的方框内容。