从大肠杆菌中纯化His标签融合蛋白。

Purification of His-Tag fusion proteins from Escherichia coli.

作者信息

Hengen P

机构信息

National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

出版信息

Trends Biochem Sci. 1995 Jul;20(7):285-6. doi: 10.1016/s0968-0004(00)89045-3.

DOI:10.1016/s0968-0004(00)89045-3

PMID:7667882

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.

摘要

《方法与试剂》是一个独特的月度专栏，重点介绍互联网上新闻组bionet.molbio.methds - reagnts中当前的讨论内容。本月专栏讨论了在通过金属螯合亲和层析纯化组氨酸标签融合蛋白时发现的一种来自大肠杆菌的污染蛋白。有关如何参与新闻组的详细信息，请参阅随附的方框内容。