Houpert P, Serthelon J P, Lefebvre H P, Toutain P L, Braun J P
Departement de Physiopathologie, Ecole Nationale Veterinaire, Toulouse, France.
Vet Hum Toxicol. 1995 Apr;37(2):105-10.
Intramuscular drug administration can lead to more or less extensive muscle damage. The aim of the present study was to show the possibility of quantitating, in vivo and non-invasively, the equivalence of muscle destroyed by the administration of a test drug (phenylbutazone) known for its injurious properties. Creatine kinase (CK) kinetic parameters (clearance, volume of distribution) were measured in 6 sheep after an iv administration of muscle CK homogenate. In the same 6 sheep, CK release after iv and im 8 mg phenylbutazone/kg was measured. The calculated total CK released, based on the CK plasma clearance (0.28 mL/kg/min) and area under the curve of CK activity after im phenylbutazone administration was 191 +/- 140 U/kg. By relating this quantity to that of CK gluteal muscle (5114 +/- 891 U/g), it was calculated that im phenylbutazone administration was able to destroy an equivalence of 2.4 +/- 2.1 g of muscle. For the 2 main sites of im administration (neck and gluteal muscle), general equations are proposed to calculate the equivalence of muscle destroyed in sheep when only plasma CK activity following a test drug administration is available.
肌肉注射药物可能会或多或少地导致广泛的肌肉损伤。本研究的目的是展示在体内和非侵入性地定量因注射具有损伤特性的受试药物（保泰松）而破坏的肌肉等效量的可能性。在静脉注射肌肉肌酸激酶（CK）匀浆后，测量了6只绵羊的CK动力学参数（清除率、分布容积）。在同一6只绵羊中，测量了静脉注射和肌肉注射8mg保泰松/千克体重后的CK释放量。根据CK血浆清除率（0.28mL/千克体重/分钟）和肌肉注射保泰松后CK活性曲线下面积计算得出的总CK释放量为191±140U/千克体重。通过将该量与臀肌的CK量（5114±891U/克）相关联，计算得出肌肉注射保泰松能够破坏相当于2.4±2.1克的肌肉。对于肌肉注射的两个主要部位（颈部和臀肌），当仅获得受试药物给药后的血浆CK活性时，提出了通用方程来计算绵羊中被破坏的肌肉等效量。
