Lefebvre H P, Toutain P L, Bret L, Rico A G, Braun J P
INRA, Département de Physiopathologie et URA, Toulouse, France.
Vet Res. 1993;24(6):468-76.
The purpose of this study was to compare the disposition parameters of creatine kinase (CK) in rabbits after intravenous bolus administration of 3 CK preparations: 1) purified CK; 2) CK obtained from a muscular extract after a 3,000 g centrifugation (ie with cell remains) (3,000 g CK); or 3) a 105,000 g centrifugation (ie the cytosolic soluble phase) (105,000 g CK). The plasma half-lives were not significantly different (approximately 9 h). In contrast, the clearance of 3,000 g CK (3.25 +/- 0.33 ml.kg-1.h-1) was significantly lower than that of the 2 others (7.00 +/- 0.49 ml.kg-1.h-1 and 4.63 +/- 0.65 ml.kg-1.h-1 for 105,000 g and purified CK, respectively). These findings suggest that purified CK preparation is not the most appropriate form for determination of enzyme pharmacokinetic parameters following muscle damage.
本研究的目的是比较家兔静脉注射大剂量3种肌酸激酶（CK）制剂后的处置参数：1）纯化的CK；2）经3000g离心（即含有细胞残余物）后从肌肉提取物中获得的CK（3000g CK）；或3）经105000g离心（即胞质可溶性部分）后获得的CK（105000g CK）。血浆半衰期无显著差异（约9小时）。相比之下，3000g CK的清除率（3.25±0.33 ml·kg-1·h-1）显著低于其他两种制剂（105000g CK和纯化CK的清除率分别为7.00±0.49 ml·kg-1·h-1和4.63±0.65 ml·kg-1·h-1）。这些发现表明，纯化的CK制剂并非测定肌肉损伤后酶药代动力学参数的最合适形式。
