贝克曼阵列上人类IgG亚类检测方法的评估

Evaluation of human IgG subclass assays on Beckman array.

作者信息

Pressac M, Allouche F, Circaud R, Aymard P

机构信息

Laboratoire de Biochimie, Hôpital Armand Trousseau, Paris, France.

出版信息

Ann Clin Biochem. 1995 May;32 ( Pt 3):281-8. doi: 10.1177/000456329503200304.

DOI:10.1177/000456329503200304

PMID:7632032

Abstract

Automated immunonephelometric assays were developed to measure human IgG subclasses in serum, on the Beckman Array Protein System (APSR), with sheep antihuman IgG subclass antisera. The interassay imprecision was judged to be satisfactory in each case (CV: IgG1: 2.3%; IgG2: 2.9%; IgG3: 4.0% and IgG4 5.4%). The standard curves were found to be linear in the ranges 1.5-30.0 g/L for IgG1, 1.5-15.0 g/L for IgG2, 0.1-1.0 g/L for IgG3 and 0.1-1.0 g/L for IgG4. The lower limit for quantification of the immunephelometric assays was 0.5 g/L for IgG1, 0.04 g/L for IgG2, 0.06 g/L for IgG3 and 0.03 g/L for IgG4. We found no antigen excess to at least 60.0 g/L for IgG1 and IgG2, 3.5 g/L for IgG3 and 2.0 g/L for IgG4. Nephelometric results correlated with those of radial immunodiffusion (r = 0.96 for IgG1, r = 0.93 for IgG2, r = 0.90 for IgG3 and r = 0.96 for IgG4). The reaction monitored by the Beckman Array Protein System is easy to perform, rapid and precise. The performance makes the immunonephelometric method suitable for the determination of IgG subclass concentrations in routine analysis.

摘要

采用羊抗人IgG亚类抗血清，在贝克曼Array Protein System（APSR）上开发了自动免疫比浊法，用于检测血清中的人IgG亚类。各情况下批间不精密度均令人满意（变异系数：IgG1为2.3%；IgG2为2.9%；IgG3为4.0%；IgG4为5.4%）。发现标准曲线在以下范围内呈线性：IgG1为1.5 - 30.0 g/L，IgG2为1.5 - 15.0 g/L，IgG3为0.1 - 1.0 g/L，IgG4为0.1 - 1.0 g/L。免疫比浊法的定量下限为：IgG1为0.5 g/L，IgG2为0.04 g/L，IgG3为0.06 g/L，IgG4为0.03 g/L。对于IgG1和IgG2，至少在60.0 g/L时未发现抗原过量；对于IgG3，在3.5 g/L时未发现抗原过量；对于IgG4，在2.0 g/L时未发现抗原过量。比浊法结果与放射免疫扩散法结果相关（IgG1的r = 0.96，IgG2的r = 0.93，IgG3的r = 0.90，IgG4的r = 0.96）。贝克曼Array Protein System监测的反应易于操作、快速且精确。该性能使免疫比浊法适用于常规分析中IgG亚类浓度的测定。