Laughner B, Lawrence S D, Ferl R J
Department of Horticultural Sciences, University of Florida, Gainesville 32611, USA.
Biochim Biophys Acta. 1995 Jul 25;1263(1):67-70. doi: 10.1016/0167-4781(95)00092-u.
Two tomato cDNA clones with homology to the 14-3-3 family of proteins were identified through immunoscreening a ripening tomato fruit library (Clontech). These two clones share approx. 71% identity at the nucleotide level and 84% identity at the deduced amino acid level, with radical amino acid substitutions clustering at the acidic carboxy-terminus. Southern hybridization data indicate that each clone represents a unique gene. Distinct transcript accumulation patterns during tomato fruit ripening together with the homology to brain regulatory proteins suggest potential involvement in fruit development.
通过对成熟番茄果实文库（Clontech公司）进行免疫筛选，鉴定出两个与14-3-3蛋白家族具有同源性的番茄cDNA克隆。这两个克隆在核苷酸水平上约有71%的同一性，在推导的氨基酸水平上有84%的同一性，酸性羧基末端聚集着氨基酸的根本替换。Southern杂交数据表明每个克隆代表一个独特的基因。番茄果实成熟过程中不同的转录物积累模式以及与脑调节蛋白的同源性表明其可能参与果实发育。
