糖缀合物的合成与筛选策略。2. 用于流式细胞术的共价固定化。

Strategies for the synthesis and screening of glycoconjugates. 2. Covalent immobilization for flow cytometry.

作者信息

Vetter D, Tate E M, Gallop M A

机构信息

Affymax Research Institute, Palo Alto, California 94304, USA.

出版信息

Bioconjug Chem. 1995 May-Jun;6(3):319-22. doi: 10.1021/bc00033a014.

DOI:10.1021/bc00033a014

PMID:7632806

Abstract

Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.

摘要

糖基胺是易于获得的碳水化合物衍生物，可与同双功能N-羟基琥珀酰亚胺酯发生酰化反应。产物糖基酰胺带有一个配备有一个活性酯官能团的间隔基团。该途径提供了定义明确的糖缀合物，其可与各种氨基官能化树脂交联。通过使用荧光标记的凝集素进行凝集素免疫染色或流式细胞术来探测所得糖珠缀合物的碳水化合物识别。