Dwyer B E, Nishimura R N, Lu S Y
Molecular Neurobiology Laboratory (IIIN-1), Department of Veterans Affairs Medical Center, Sepulveda, CA 91343, USA.
Neuroreport. 1995 May 9;6(7):973-6. doi: 10.1097/00001756-199505090-00006.
Western blot analysis using several antibodies showed that rat spinal cord contained abundant immunostainable heme oxygenase-2 (HO-2) and barely detectable levels of heme oxygenase-1 (HO-1). Anti-HO-2 antibody stained large anterior horn motoneurones and numerous smaller neurons throughout spinal cord gray matter including the dorsal root entry zone. HO-2+ astrocytes were not evident in gray matter although their presence cannot be ruled out. The distribution of HO-2+ neurons was compared with the distribution of cells containing NADPH-diaphorase (NADPH-d) activity, a marker for nitric oxide synthase. NADPH-d activity was restricted to far fewer neurons, many of which were close to the central canal and dorsal root entry zone.
使用多种抗体进行的蛋白质免疫印迹分析表明，大鼠脊髓含有大量可免疫染色的血红素加氧酶-2（HO-2），而血红素加氧酶-1（HO-1）的水平几乎检测不到。抗HO-2抗体对整个脊髓灰质（包括背根进入区）中的大型前角运动神经元和众多较小的神经元进行了染色。尽管不能排除HO-2+星形胶质细胞的存在，但在灰质中并不明显。将HO-2+神经元的分布与含有烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）活性的细胞分布进行了比较，NADPH-d活性是一氧化氮合酶的标志物。NADPH-d活性仅限于少得多的神经元，其中许多靠近中央管和背根进入区。
