Berliz G, Wintersteiger R, Pacha W
EBEWE Pharmaceuticals, Unterach/Attersee, Austria.
J Chromatogr B Biomed Appl. 1995 Apr 21;666(2):368-73. doi: 10.1016/0378-4347(94)00592-s.
A reversed-phase liquid chromatographic method with coulometric detection has been developed for the determination of free MKS 213-492 (a novel xanthine derivative) in plasma. This method is based on a simple and rapid plasma extraction procedure using precolumn-switching. The oxidation of this pharmacologically active xanthine derivative was optimized with respect to applied potential, pH of mobile and rinsing phase and rinsing time. The detection limit for MKS 213-492 was found to be 54 pg/injection.
已开发出一种采用库仑检测的反相液相色谱法，用于测定血浆中游离的MKS 213-492（一种新型黄嘌呤衍生物）。该方法基于使用柱前切换的简单快速血浆萃取程序。针对施加电位、流动相和冲洗相的pH值以及冲洗时间，对这种具有药理活性的黄嘌呤衍生物的氧化进行了优化。发现MKS 213-492的检测限为54 pg/进样。
