Akiyama K, Ono M, Kohira I, Daigen A, Ishihara T, Kuroda S
Department of Neuropsychiatry, Okayama University Medical School, Japan.
Brain Res. 1995 May 15;679(2):212-20. doi: 10.1016/0006-8993(95)00221-b.
Previous studies have demonstrated that membrane-associated protein kinase C (PKC) activities in the right and left hippocampus of rats kindled from the left hippocampus increased significantly at 4 weeks [9] and 4 months [22] after the last seizure compared with those in matched control rats. In this study, we investigated the effect of kindling from the left amygdala on PKC activities in the amygdala/pyriform cortex and hippocampus at long seizure-free intervals (4 and 16 weeks) from the last amygdala-kindled seizure. Membrane-associated PKC activity of the kindled group increased significantly only in the left hippocampus compared with the left side control (the left hippocampus of rats subjected to a sham operation) at 4 weeks (by 34%, P < 0.03) and 16 weeks (by 24%, P < 0.05) after the last seizure. There was no significant alteration in the membrane-associated PKC activity of the kindled group in the right hippocampus or amygdala/pyriform cortex in any seizure-free interval after the last amygdala seizure. Cytosolic PKC activity did not differ between the kindled and control groups in any brain region examined in any seizure-free interval. At 16 weeks after the last seizure, the PKC activity in the P1 fraction of the kindled group increased significantly only in the left hippocampus (by 49%, P < 0.005), but not in the right hippocampus. Neither PKC activity in the P2 fraction nor that in the cytosolic fraction was altered in the kindled group after this seizure-free interval.(ABSTRACT TRUNCATED AT 250 WORDS)
先前的研究表明，与匹配的对照大鼠相比，从左海马点燃的大鼠在末次癫痫发作后4周[9]和4个月[22]时，其左右海马中与膜相关的蛋白激酶C（PKC）活性显著增加。在本研究中，我们调查了从左杏仁核点燃对末次杏仁核点燃癫痫发作后长时间无癫痫发作间隔（4周和16周）时杏仁核/梨状皮质和海马中PKC活性的影响。与左侧对照（假手术大鼠的左海马）相比，点燃组的膜相关PKC活性仅在末次癫痫发作后4周（增加34%，P<0.03）和16周（增加24%，P<0.05）时在左海马中显著增加。在末次杏仁核癫痫发作后的任何无癫痫发作间隔期间，点燃组右侧海马或杏仁核/梨状皮质中的膜相关PKC活性均无显著变化。在任何无癫痫发作间隔期间，点燃组和对照组在任何检查的脑区中，胞质PKC活性均无差异。在末次癫痫发作后16周，点燃组P1组分中的PKC活性仅在左海马中显著增加（增加49%，P<0.005），而在右海马中未增加。在这个无癫痫发作间隔期后，点燃组的P2组分和胞质组分中的PKC活性均未改变。（摘要截短为250字）
