Fankhauser C, Reymond A, Cerutti L, Utzig S, Hofmann K, Simanis V
Swiss Institute for Experimental Cancer Research.
Cell. 1995 Aug 11;82(3):435-44. doi: 10.1016/0092-8674(95)90432-8.
The S. pombe cdc15 gene is essential for cell division. cdc15ts mutants do not form a septum, but growth and nuclear division continue, leading to formation of multinucleate cells. The earliest step in septum formation and cytokinesis, rearrangement of actin to the center of the cell, is associated with appearance of hypophosphorylated cdc15p and formation of a cdc15p ring, which colocalizes with actin. Loss of cdc15p function impairs formation of the actin ring. The abundance of cdc15 mRNA varies through the cell division cycle, peaking in early mitosis before septation. Expression of cdc15 in G2-arrested cells induces actin rearrangement to the center of the cell. These data implicate cdc15p as a key element in mediating the cytoskeletal rearrangements required for cytokinesis.
粟酒裂殖酵母的cdc15基因对细胞分裂至关重要。cdc15温度敏感型突变体不形成隔膜，但生长和核分裂仍在继续，导致多核细胞的形成。隔膜形成和胞质分裂的最早步骤，即肌动蛋白重排到细胞中心，与低磷酸化cdc15p的出现和cdc15p环的形成相关，该环与肌动蛋白共定位。cdc15p功能的丧失会损害肌动蛋白环的形成。cdc15 mRNA的丰度在细胞分裂周期中有所变化，在有丝分裂早期隔膜形成之前达到峰值。在G2期停滞的细胞中表达cdc15会诱导肌动蛋白重排到细胞中心。这些数据表明cdc15p是介导胞质分裂所需细胞骨架重排的关键元件。
