Yokoyama K, Nojiri H, Suzuki M, Setaka M, Suzuki A, Nojima S
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
FEBS Lett. 1995 Jul 24;368(3):477-80. doi: 10.1016/0014-5793(95)00714-k.
Although the ceramide components of both glycosphingolipids (GSLs) and sphingomyelin (SM) in HL-60 cells were identical, the molecular species of the ceramides preferentially used in biosynthesis were quite different in GSLs and SM. When HL-60 cells were stimulated to differentiate into macrophage-like cells by phorbol ester after their sphingolipids had been metabolically labeled with L-[3-14C]serine to saturation point, marked changes in the radioactivities of the ceramide residues were observed in GSLs, showing the activation of a biosynthetic pathway of ganglioside GM3. No significant changes were, however, observed in the ceramide residues of SM. These results indicate that it is necessary to consider the overall metabolism of ceramides, including their origin, when investigating the functions of ceramides in signal transduction systems.
尽管HL-60细胞中糖鞘脂（GSLs）和鞘磷脂（SM）的神经酰胺成分相同，但在生物合成中优先使用的神经酰胺分子种类在GSLs和SM中却有很大差异。在用L-[3-14C]丝氨酸将HL-60细胞的鞘脂代谢标记至饱和点后，用佛波酯刺激其分化为巨噬细胞样细胞，结果发现GSLs中神经酰胺残基的放射性有显著变化，表明神经节苷脂GM3的生物合成途径被激活。然而，SM的神经酰胺残基未观察到明显变化。这些结果表明，在研究神经酰胺在信号转导系统中的功能时，有必要考虑神经酰胺的整体代谢，包括其来源。
