Kiguchi K, Henning-Chubb C, Huberman E
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
Biochim Biophys Acta. 1993 Mar 10;1176(1-2):27-36. doi: 10.1016/0167-4889(93)90173-m.
The patterns of glycosphingolipids (GSLs) were analyzed in an HL-60 cell variant, HL-205, which is susceptible to phorbol 12-myristate 13-acetate (PMA)-induced monocyte/macrophage differentiation, and in an HL-60 cell variant, HL-525, which is resistant to such differentiation. The amounts and types of the GSLs were similar in both the HL-205 and HL-525 cells and they resemble those of granulocytes. Treatment with 3 nM PMA caused the susceptible HL-205 cells (but not the resistant cells) to acquire a new GSL pattern which resembles that of monocytes. This new pattern was characterized by increases in the level of a neutral GSL, Gb3Cer, from trace levels to 0.05 mg/10(9) cells and of an acidic GSL, GM3 ganglioside, from 0.03 to 0.33 mg/10(9) cells. The increases in the level of this ganglioside were found to be due to an increase in CMP-N-acetylneuraminic acid:lactosylceramide sialyltransferase activity. These results indicate an association between PMA-induced terminal differentiation along the monocyte/macrophage cell lineage and PMA-evoked increases in specific GSLs, GM3 in particular, which is due to a rise in the activity of its synthetic enzyme.
在一种对佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）诱导的单核细胞/巨噬细胞分化敏感的HL - 60细胞变体HL - 205以及一种对这种分化具有抗性的HL - 60细胞变体HL - 525中分析了糖鞘脂（GSLs）的模式。HL - 205和HL - 525细胞中GSLs的数量和类型相似，且与粒细胞的相似。用3 nM PMA处理导致敏感的HL - 205细胞（但不包括抗性细胞）获得一种类似于单核细胞的新GSL模式。这种新模式的特征是一种中性GSL，Gb3Cer的水平从痕量增加到0.05 mg/10⁹细胞，以及一种酸性GSL，GM3神经节苷脂的水平从0.03增加到0.33 mg/10⁹细胞。发现这种神经节苷脂水平的增加是由于CMP - N - 乙酰神经氨酸：乳糖基神经酰胺唾液酸转移酶活性的增加。这些结果表明沿着单核细胞/巨噬细胞细胞谱系的PMA诱导的终末分化与PMA引起的特定GSLs增加之间存在关联，特别是GM3，这是由于其合成酶活性的升高。
