Gunin A
Department of Histology Medical Faculty, Shuvash State University, Cheboksary, Russia.
Eur J Obstet Gynecol Reprod Biol. 1995 May;60(1):69-74. doi: 10.1016/0028-2243(95)02074-8.
The aim of this study was to examine the influence of acute stress on the realization of the effects of estrogens in the uterus of ovariectomized rats. The rats were immobilized for 6 h and given a single injection of estradiol dipropionate (E2; 10 micrograms/rat, i.m.). E2-only treated rats, olive oil injected and stressed rats, olive oil-only treated rats and untreated ovariectomized animals were used as controls. The effect of E2 was assessed by the volumes of cells, nuclei and nucleouli (morphometry), DNA content (Feulgen technique) and proliferative activity (mitotic index) in luminal epithelium, in glandular epithelium and in stromal cells of endometrium 24, 36 and 48 h after the injection of E2 or olive oil. All the effects of E2 were reduced in E2-treated rats subjected to stress in all the uterine structures. In olive oil treated rats, stress induced certain increase in the volume of cells and nuclei and slight increase in the DNA content in all the structures. It is suggested that the influence of stress on the uterus is mediated by impairment of some septs in the mechanism of action of estrogen on the uterus, which leads to a decrease in the sensitivity of the uterine structures to estrogens. Without estrogen stimulation the effect of stress on the uterus is realized via estrogen-independent pathways.
本研究的目的是考察急性应激对去卵巢大鼠子宫中雌激素效应实现的影响。将大鼠固定6小时，并单次注射二丙酸雌二醇（E2；10微克/只，肌肉注射）。仅用E2处理的大鼠、注射橄榄油并应激的大鼠、仅用橄榄油处理的大鼠以及未处理的去卵巢动物用作对照。在注射E2或橄榄油后24、36和48小时，通过测量子宫内膜腔上皮、腺上皮和基质细胞中的细胞、细胞核及核仁体积（形态测量法）、DNA含量（福尔根技术）和增殖活性（有丝分裂指数）来评估E2的作用效果。在所有子宫结构中，遭受应激的E2处理大鼠中E2的所有作用效果均降低。在橄榄油处理的大鼠中，应激导致所有结构中的细胞和细胞核体积有一定增加，DNA含量略有增加。提示应激对子宫的影响是通过损害雌激素对子宫作用机制中的某些环节介导的，这导致子宫结构对雌激素的敏感性降低。在没有雌激素刺激的情况下，应激对子宫的影响通过雌激素非依赖途径实现。
