Gunin A G, Sharov A A
Department of Histology, Medical Institute of Chuvash State University, Cheboksary, Russia.
J Reprod Fertil. 1998 May;113(1):61-8. doi: 10.1530/jrf.0.1130061.
The role of mast cells and their main secretory products in the effects of oestradiol on the uterus was investigated. Ovariectomized rats were treated with a single injection of oestradiol (10 micrograms per rat, i.m.) or vehicle together with drugs affecting the activity of mast cells, cromoglycate (10 mg per rat, i.m.), which diminishes the degranulation of mast cells, or compound 48/80 (0.5 mg per rat, i.m.), which enhances this process. Oestradiol or vehicle was also administered with two important secretory products of mast cells, heparin (0.4 mg per rat, i.m.) or histamine (2 mg per rat, i.m.). All drugs were injected simultaneously with oestradiol (first injection) and then every 6 h until the animals were killed. Observations were performed at 24, 36 and 48 h after oestradiol or vehicle injection. The condition of mast cells was determined by the percentage of degranulated mast cells in sections stained with toluidine blue. Oestradiol-induced effects in the uterus were estimated by the mitotic index, proliferating cell nuclear antigen-labelling index, DNA content, volumes of cells, nuclei and nucleoli in the luminal epithelium, glandular epithelium and stroma cells of the endometrium. Cromoglycate treatment resulted in a decrease in both mast cell degranulation and all examined oestradiol effects in the uterus at all periods of observation. Compound 48/80 increased mast cell degranulation and expression of one aspect of oestradiol effects on the volumes of cell compartments. Histamine or heparin led to a marked increase in the cell, nucleus and nucleolus volumes in all uterine structures. However, heparin produced a depression in proliferation, whereas histamine had a weak transient stimulating action on this process. No effects of the protocols were found in the absence of oestradiol treatment. These results suggest that mast cells are involved in the realization of oestrogen action, including the stimulation of cell growth and proliferation in the uterus, and that the effect of mast cells is mediated by both histamine and heparin.
研究了肥大细胞及其主要分泌产物在雌二醇对子宫作用中的角色。对去卵巢大鼠单次注射雌二醇（每只大鼠10微克，肌肉注射）或溶剂，并同时给予影响肥大细胞活性的药物，如色甘酸（每只大鼠10毫克，肌肉注射），它可减少肥大细胞脱颗粒，或化合物48/80（每只大鼠0.5毫克，肌肉注射），它可增强这一过程。雌二醇或溶剂还与肥大细胞的两种重要分泌产物肝素（每只大鼠0.4毫克，肌肉注射）或组胺（每只大鼠2毫克，肌肉注射）一起给药。所有药物均与雌二醇（首次注射）同时注射，然后每6小时注射一次，直至动物被处死。在注射雌二醇或溶剂后24、36和48小时进行观察。通过用甲苯胺蓝染色切片中脱颗粒肥大细胞的百分比来确定肥大细胞的状态。通过有丝分裂指数、增殖细胞核抗原标记指数、DNA含量、子宫内膜腔上皮、腺上皮和基质细胞中的细胞、细胞核和核仁体积来评估雌二醇对子宫的诱导作用。色甘酸治疗导致在所有观察期肥大细胞脱颗粒以及子宫中所有检测的雌二醇作用均降低。化合物48/80增加了肥大细胞脱颗粒以及雌二醇对细胞区室体积作用的一个方面的表达。组胺或肝素导致所有子宫结构中的细胞、细胞核和核仁体积显著增加。然而，肝素导致增殖受到抑制，而组胺对这一过程有微弱的短暂刺激作用。在未进行雌二醇治疗的情况下未发现这些方案有任何作用。这些结果表明肥大细胞参与雌激素作用的实现，包括刺激子宫中的细胞生长和增殖，并且肥大细胞的作用由组胺和肝素介导。
