Gunin A G
Department of Histology, Medical Institute of Chuvash State University, Cheboksary, Russia.
J Reprod Fertil. 1997 Jul;110(2):299-306. doi: 10.1530/jrf.0.1100299.
The aim of this study was to examine the effect of growth hormone on the uterine response to oestradiol. Ovariectomized rats were treated with a single injection of oestradiol dipropionate (10 micrograms per rat, i.m.), together with a single injection of human growth hormone (0.04 mg per rat, i.m.). The effects of oestradiol in the uterus were determined by measuring the following: mitotic index; the percentage of cells positive for the proliferating cell nuclear antigen (using immunocytochemistry); the DNA content (using Feulgen's method); and the volume of cells, nuclei and nucleoli (using morphometry) in the luminal epithelium, glandular epithelium and stroma of the endometrium 24, 36 and 48 h after injection with oestradiol. All values for all structures were decreased after oestradiol and growth hormone treatment compared with values of rats treated with oestradiol only. However, parameters that reflected proliferative processes (mitotic indices, the number of cells positive for proliferating cell nuclear antigen, and DNA content) were much more reduced than those indicating cell growth (volumes of cells, nuclei and nucleoli). In the absence of oestradiol, administration of growth hormone did not influence the uterine parameters tested. The effect of growth hormone is likely to be brought about by changing the mechanism of oestrogen action on the uterus.
本研究的目的是检测生长激素对子宫对雌二醇反应的影响。对去卵巢大鼠单次注射二丙酸雌二醇（每只大鼠10微克，肌肉注射），同时单次注射人生长激素（每只大鼠0.04毫克，肌肉注射）。通过测量以下指标来确定雌二醇对子宫的影响：有丝分裂指数；增殖细胞核抗原阳性细胞的百分比（采用免疫细胞化学法）；DNA含量（采用福尔根氏法）；以及在注射雌二醇后24、36和48小时，子宫内膜腔上皮、腺上皮和基质中细胞、细胞核和核仁的体积（采用形态测量法）。与仅接受雌二醇治疗的大鼠相比，雌二醇和生长激素治疗后所有结构的所有值均降低。然而，反映增殖过程的参数（有丝分裂指数、增殖细胞核抗原阳性细胞数量和DNA含量）比表明细胞生长的参数（细胞、细胞核和核仁的体积）降低得更多。在没有雌二醇的情况下，生长激素的给药不影响所检测的子宫参数。生长激素的作用可能是通过改变雌激素对子宫的作用机制来实现的。
